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用表达gfp标记的细胞色素C氧化酶VIII的慢病毒颗粒感染HUVECs。选用耐嘌呤霉素的线粒体- gfp - huvecs并在冷冻瓶中运输(提供P3代的细胞)。建议使用内皮生长培养基进行细胞培养,按照推荐方法培养时,细胞可以达到18倍增殖。
HUVECs were infected with Lentiviral particles expressing GFP-Tagged Cytochrome C oxidase VIII @ passage 1. Puromycin resistant Mitochondria-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).
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