人脐静脉内皮细胞-质膜GFP标记

用表达gfp标记的神经调节蛋白的慢病毒颗粒感染HUVECs。我们选择耐嘌呤霉素的PM-GFP-HUVECs，并在冷冻瓶中运输(提供P3代细胞)。建议使用内皮生长培养基进行细胞培养，按照推荐的方案培养时，细胞可达到18倍增数。

HUVECs were infected with Lentiviral particles expressing GFP-Tagged neuromodulin @ passage 1. Puromycin resistant PM-GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).

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