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用表达RFP的慢病毒颗粒在P1代感染HUVECs。我们选择耐Zeocin的rpm - huvecs,并将其装在冷冻瓶中(提供P3代的细胞)。推荐使用内皮生长培养基进行细胞培养,按照推荐方案培养时,细胞可达到18倍增。
HUVECs were infected with RFP- expressing Lentiviral particles @ passage 1. Zeocin resistant RPF-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).
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