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从表达GFP慢病毒颗粒的HGMVECs中筛选出对嘌呤霉素耐药的HGMVECs。细胞以冷冻瓶运输(提供P4-P5代细胞)。建议使用内皮生长培养基进行细胞培养,按照推荐方案培养时,细胞最少可达到18倍增次数。
GFP-HGMVECs were selected from HGMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels > 18 when cultured following the detailed protocol described below.
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