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从表达RFP慢病毒颗粒的HGMVECs感染对Zeocin耐药的HGMVECs中筛选出RFP-HGMVECs。细胞以冷冻瓶运输(提供P4-P5代细胞)。建议使用内皮生长培养基进行细胞培养,按照推荐详细方案培养时,细胞可达到18倍增。
RFP-HGMVECs were selected from HGMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels > 18 when cultured following the detailed protocol described below.
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