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从表达GFP慢病毒颗粒的HAAECs中筛选出GFP-HAAECs。细胞在P3代冻存。推荐使用内皮生长培养基进行细胞培养,按照推荐方案培养时,细胞可达14倍增。
GFP-HAAECs were selected from HAAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels > 14 when cultured following the detailed protocol described below.
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