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从表达GFP慢病毒颗粒的HRMVECs中筛选出对purocin耐药的HRMVECs。细胞冷冻(提供P5代细胞)。推荐使用内皮生长培养基进行细胞培养,当按照推荐方案培养时,细胞可达到16倍增。
GFP-HRMVECs were selected from HRMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels > 16 when cultured following the detailed protocol described below.
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