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当前位置：首页 > 产品中心(Products) > 细胞定制Cell Customization(Cell Customization) > 稳转细胞株(Transfected Stable Cell Lines) > 示踪/报告细胞株(Report Transfection cell line) > 人皮肤成纤维细胞-GFP/LUC双标(新生儿）GFP-Luc-HNDFCs-Neo
产品描述/Products Description
从正常新生儿包皮组织中分离出HNDFCs-Neo细胞，并在P1代转染表达GFP和荧光素酶的慢病毒颗粒。选择耐嘌呤霉素的GFP-Luc-HNDFCs-Neo细胞，在P3代进行冻存运输。建议使用成纤维细胞生长培养基进行细胞培养，当按照推荐详细方案进行培养时，细胞可达14倍增。
HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with GFP and Luciferase expressing Lentiviral particles at passage one. Puromycin resistant GFP-Luc-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels > 14 when cultured following the detailed protocol described below.
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