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从正常人肺组织(成人)样本中分离出HLFCs-Ad,用表达GFP的慢病毒感染HLFCs-Ad后,用嘌呤霉素筛选GFP-HLFCs-Ad细胞,细胞在P2代冻存提供。建议使用通用生长培养基进行细胞培养,当按照推荐详细方案进行培养时,细胞可达10倍增。
HLFCs-Ad are isolated from normal human lung tissue(adult) samples and GFP-HLFCs-Ad cells are selected by puromycin after infection HLFCs-Ad with lentiviruses expressing GFP and shipped in frozen vial (the cells are provided @ passage 2). Universal Growth Medium is recommended for cell culture and these cells have an average minimum population doubling levels > 10 when cultured following the detailed protocol described below.
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