人皮肤成纤维细胞-新生儿（HDF）

正常人皮肤成纤维细胞（HDF）为建立无血清的人胚胎干细胞饲养层或作为研究创伤愈合、毒理学或基础细胞生物学的模型提供了理想的细胞系统。成纤维细胞作为原代细胞被冷冻保存，以确保最高的活性和纯度。我们的成纤维细胞在LIFib无血清培养基和LIFib S2培养基中都进行了培养测试，以确保细胞在经典血清补充培养基的速率实现最佳生长。细胞特征：HDFn作为原代细胞被冷冻保存。从新生儿包皮中分离细胞，在冷冻保存前在培养血管中扩增一次。原代HDFa细胞作为冷冻保存。细胞从成人皮肤中分离出来，在冷冻保存前在培养血管中扩增一次。将人肺成纤维细胞冷冻保存，将人肺成纤维细胞从人肺中分离出来，在冻存前在培养血管中扩增两次。成纤维细胞可以在无血清、无动物蛋白、无酚红和无抗菌剂的条件下生长。成纤维细胞经过广泛的测试，以达到质量标准并表现出最佳性能。成纤维细胞适合用作人胚胎干细胞的无氙或非氙培养的饲养层，以及其他需要饲养层的细胞培养应用。

normal Human Dermal Fibroblasts (HDF) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our fibroblasts are quality tested in both LIFib Serum-Free Medium and LIFib S2 Medium to ensure optimal growth at rates equal to or greater than classical serum-supplemented medium. Cell Features: HDFn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded in culture vessels once before cryopreservation. HDFa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded in culture vessels once before cryopreservation. Human Lung Fibroblasts are cryopreserved as secondary cells cells are isolated from human lungs and expanded in culture vessels twice before cryopreservation. Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium. Fibroblasts are extensively tested to meet quality standards and exhibit optimal performance. Fibroblasts are suitable for use as feeder layers as xeno-free or non-xeno free cultures for human embryonic stem cells and other cell culture applications requiring feeder layers.

For research use only