人包皮成纤维细胞(囊性纤维化患者成纤维细胞)

背景介绍/context：

iPSC (induced Pluripotent Stem cells) reprogrammed from human Fibroblasts of a cystic fibrosis patient.This iPS cell line was created using a normal human fibroblast of a male adult patient suffering from Cystic Fibrosis. The iPS cell line was generated using an episomal reprogramming containing a prprietary mix of vectors, containing Oct-4, Sox-2, Klf-4 along with p53 Anti-sense, EBNA-1.The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4.iPS colonies exhibit classical morphology and growth.Absence of Mycoplasma was confirmed.The cell line is shipped on dry ice.

由囊性纤维化患者的人类成纤维细胞重新编程的诱导多能干细胞。这个iPS细胞系是用一个患有囊性纤维化的成年男性患者的正常人类成纤维细胞创造的。iPS细胞系是通过一种非遗传重编程产生的，其中含有一种彗星载体组合，包括Oct-4, Sox-2, Klf-4和p53 Anti-sense, EBNA-1。根据菌落形态、碱性磷酸酶表达和SSEA-4表达验证该细胞株的多能性。iPS菌落呈现典型的形态和生长。证实无支原体。细胞系用干冰运送。

动物种别/Organism  人

组织来源 Tissue and Cell Type 诱导多能干细胞

形态/Morphology 贴壁生长 

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