来自成纤维细胞/男性/逆转录病毒的IPSC)

背景介绍/context：

Human iPS (induced pluripotent stem) cell line was derived from human foreskin fibroblasts (HFFs) by retroviral expression of OCT4, SOX2, KLF4, and c-MYC genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and

Nanog, and demonstrate strong endogenous alkaline phosphatase activity. The low passage iPS cells are highly viable and have been pre-adapted to serum-free,

feeder-free culture conditions.

人iPS (induced pluripotent stem, induced iPS)细胞系来源于人包皮成纤维细胞(Human包皮成纤维细胞，HFFs)，通过逆转录病毒表达OCT4、SOX2、KLF4和c-MYC基因。细胞的来源使用形态学选择标准，没有使用荧光标记或药物选择。在标准的人类胚胎干细胞培养条件下培养，人类iPS细胞的形态与人类胚胎干细胞相同。这些细胞还表达了多能性标记SSEA-3和Nanog，并表现出很强的内源性碱性磷酸酶活性。低传代诱导多能干细胞具有很高的存活能力，并且已经预先适应了无血清、无刺激的培养条件。

动物种别/Organism  人

组织来源 Tissue and Cell Type iPS细胞

形态/Morphology 贴壁生长 

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