BALB/c小鼠肝窦内皮细胞

BALB/c小鼠肝窦内皮细胞来源于AcceGen，分离自无致病性实验小鼠组织。小鼠肝窦内皮细胞在预涂明胶溶液的T25组织培养瓶中生长0.5小时，一般在AcceGen的培养完全生长培养基中培养3-7天冻存。每瓶至少含有1x10^6细胞每毫升，干冰运输。小鼠肝窦内皮细胞可用于细胞间黏附、迁移、血管形成或跨内皮细胞阻力(TER)的测定。内皮细胞培养的标准生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫荧光染色或免疫荧光流式细胞术或产生用于所需研究应用的细胞衍生物。

BALB/c Mouse Liver Sinusoidal Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Liver Sinusoidal Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. Mouse Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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