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用表达GFP的慢病毒颗粒在P1代感染HUVECs。我们选择耐Puromycin的GFP-HUVECs,并将其装在冷冻小瓶中(提供的细胞通常是P3代)。推荐使用内皮生长培养基进行细胞培养,按照推荐方案培养时,这些细胞至少有18倍的增殖倍增水平。
HUVECs were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below.
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