人椎间盘纤维环细胞（HAFC）

椎间盘退变是腰痛的主要病理过程。腰痛的原因被认为与遗传、环境和心理社会因素有关。纤维环构成椎间盘结构的外周部分，由纤维软骨和Ⅰ、Ⅱ型胶原组成。椎间盘细胞在其局部环境中受到生物物理和机械因素的影响。白细胞介素1β可刺激纤维环细胞产生与局部降解和炎症过程有关的因子。虽然机械应力是退化的重要调节因子，但其潜在的分子机制仍不清楚。人椎间盘细胞培养为研究椎间盘退变的细胞和分子事件、组织工程和椎间盘细胞治疗提供了一种体外模型HAFC紊乱从AcceGen研究实验室分离出人类椎间盘纤维环。HAFC在原代或传代培养基中进行冷冻保存，每小瓶1毫升容量中含有>5×10^5个细胞。用免疫荧光法对HAFC进行了鉴定。HAFC细胞对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。在AcceGen研究实验室提供的条件下，HAFC可达15倍增。

Degeneration of the intervertebral disk is a major pathological process implicated in low back pain. The causes of low back pain have been suggested to involve genetic, environmental, and psychosocial factors. The anulus fibrosus makes up the peripheral portion of disk structure, composed of fibrocartilage and type I and II collagen. Cells of the intervertebral disc are influenced by both biophysical and mechanical factors in their local environment. The anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative and inflammatory processes. Although mechanical stress is an important modulator of the degeneration, the underlying molecular mechanism remains unclear. The human annulus firosus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.HAFC from AcceGen Research Laboratories are isolated from annulus fibrosus of human intervertibral disc. HAFC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by AcceGen Research Laboratories.

For research use only