L6大鼠成肌细胞

L6成肌细胞株是Yaffe从甲基胆蒽存在下大鼠大腿肌的原代培养物最初两代中分离得到。 L6细胞在培养基中融合形成多核的肌管和横纹肌纤维。 细胞融合的程度随着代数的增加而下降，因而细胞应冷冻于低代次阶段并周期性地重新克隆以选择融合能力强的细胞。 如果让培养容器中的细胞长满，这株细胞的成肌组分将迅速耗尽。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 在本库通过支原体检测。

动物种别/Organism  大鼠

组织来源/Tissue and Cell Type 骨胳肌；成肌细胞

形态/Morphology 成肌细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

     DMEM完全培养基（货号：MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS　

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用。

PubMed=5245982; DOI=10.1073/pnas.61.2.477

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