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PoASMCs-GFP cells are selected from GFP-lentiviral transfected PoASMCs resistant to PUROMYCIN. The cells are shipped in a frozen vial(the cells are provided @ passage 3). Smooth Muscle Cells(SMCs) Growth Medium is recommended for the expansion of PoASMCs-GFP and these cells can be propagated to extra 3-4 passages without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.