Neuro-2a小鼠脑神经瘤细胞

     Neuro-2A是由R.J. Klebe和F.H. Ruddle经A品系白鼠的自发肿瘤建立。取材于A品系小鼠，这个肿瘤小鼠C1300购自Jackson Laboratory。该细胞产生大量微管蛋白，此蛋白在神经细胞中起应答轴浆流动的收缩系统起作用。检测小鼠痘病毒为阴性。经无支原体鉴定入库。

组织来源 Tissue and Cell Type  脑 

形态/Morphology 神经元和阿米巴样干细胞，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions

90%MEM（MD0300）+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：MEM完全培养基（货号：MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=11470893; DOI=10.1073/pnas.151242598

Enari M., Flechsig E., Weissmann C.

Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.

Proc. Natl. Acad. Sci. U.S.A. 98:9295-9299(2001)

PubMed=14504404; DOI=10.1073/pnas.1834432100

Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.

A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.

Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

PubMed=19903493; DOI=10.1016/j.jneumeth.2009.11.004

Tremblay R.G., Sikorska M., Sandhu J.K., Lanthier P., Ribecco-Lutkiewicz M., Bani-Yaghoub M.

Differentiation of mouse Neuro 2A cells into dopamine neurons.

J. Neurosci. Methods 186:60-67(2010)

PubMed=25193168; DOI=10.1074/mcp.M113.037291

Hornburg D., Drepper C., Butter F., Meissner F., Sendtner M., Mann M.

Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Mol. Cell. Proteomics 13:3410-3420(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)