HCCLM3人肝癌细胞（高转）

该细胞取样于39岁男性患者，在本库通过STR/无支原体检测。用人肝癌细胞株MHCC97-H接种裸鼠，进行3次肺转移筛选，取肺转移瘤建成皮下接种后高度自发性肺转移的肝癌细胞系。 

动物种别/Organism 人

性别/Gender 男

组织来源 Tissue and Cell Type 肝癌

形态/Morphology  上皮细胞样,贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培养基（货号：MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=12133480

Li Y., Tang Z.-Y., Ye S.-L., Liu Y.-K., Chen J., Xue Q., Huang X.-W., Chen J., Bao W.-H., Yang J., Gao D.-M.

Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection.

Zhonghua Yi Xue Za Zhi 82:601-605(2002)

PubMed=12618900; DOI=10.1007/s00432-002-0396-4

Li Y., Tang Y., Ye L., Liu B., Liu K., Chen J., Xue Q.

Establishment of a hepatocellular carcinoma cell line with unique metastatic characteristics through in vivo selection and screening for metastasis-related genes through cDNA microarray.

J. Cancer Res. Clin. Oncol. 129:43-51(2003)

PubMed=15796966; DOI=10.1016/j.cancergencyto.2004.05.010

Yang J., Qin L.-X., Li Y., Ye S.-L., Liu Y.-K., Gao D.-M., Chen J., Tang Z.-Y.

Molecular cytogenetic characteristics of the human hepatocellular carcinoma cell line HCCLM3 with high metastatic potential: comparative genomic hybridization and multiplex fluorescence in situ hybridization.

Cancer Genet. Cytogenet. 158:180-183(2005)

PubMed=19335983

Zheng D., Liu B.-B., Liu Y.-K., Kang X.-N., Sun L., Guo K., Sun R.-X., Chen J., Zhao Y.

Analysis of the expression of Slit/Robo genes and the methylation status of their promoters in the hepatocellular carcinoma cell lines.

Zhonghua Gan Zang Bing Za Zhi 17:198-202(2009)