LMH鸡肝癌细胞

LMH是一种原发性肝细胞癌上皮细胞系，由Tomoyuki Kitagawa于1981年在日本东京都岛Kami-Ikebukuro的癌症研究所建立。用二乙基亚硝胺长期治疗长腿角鸡，在其肝脏中诱发肿瘤结节。细胞的形态是树状的。细胞表达葡萄糖-6-磷酸酶和微弱的小管ATP酶活性。这些细胞在无胸腺裸鼠体内形成肿瘤。LMH细胞含有低水平的功能性雌激素受体，并且可诱导肝脏特异性载脂蛋白II（apoII）基因的表达。

该细胞系可用于转染研究。

注：培养该细胞的培养耗材需要使用0.1%的明胶包被，或者使用基质胶包被

培养方式/Training Methods：

90%DMEM/F12(MD0600)+10%胎牛血清(SD0200)+G/A (SD0038) 

完全培养基：DMEM/F12完全培养基（货号：MD0601）

气相：空气，95%；二氧化碳，5%。 温度：33.5摄氏度

冻存条件/Cryopreservation:基础培养基+5%DMSO+20%FBS

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅供科研使用

PubMed=3607775

Kawaguchi T., Nomura K., Hirayama Y., Kitagawa T.

Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH.

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PubMed=2330000; DOI=10.1210/mend-4-2-201

Binder R., MacDonald C.C., Burch J.B.E., Lazier C.B., Williams D.L.

Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line.

Mol. Endocrinol. 4:201-208(1990)

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Efficient duck hepatitis B virus production by an avian liver tumor cell line.

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Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC).

Biochim. Biophys. Acta 1472:658-667(1999)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

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Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5

Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.

High-throughput sequencing for species authentication and contamination detection of 63 cell lines.

Sci. Rep. 11:21657-21657(2021)