CEM/C1人急性淋巴细胞白血病细胞

CEM/C1是人T细胞白血病细胞株CCRF-CEM(见ATCCCCL-119)具有喜树碱抗性的衍生株。1991年细胞株选择并亚克隆了对CPT的抗性。细胞表现出对CPT类似物水溶性的托泊替康和非水溶性的9-氨基-CPT及10,11-亚甲二氧基-CPT具有交叉抗性。CEM/C1细胞对CPT的敏感性较母系CEM细胞低31倍。CEM/C1细胞表现非典型的多药抗性和转换拓补异构酶I催化活性。对CPT的抗性维持6个月以上。经无支原体/STR鉴定入库。

动物种别/Organism 人

组织来源 Tissue and Cell Type 外周血

形态Morphology:淋巴母细胞样， 悬浮生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：1640完全培养基（货号：MD0201）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

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