C3H/10T1/2 clone8小鼠胚胎成纤维细胞

1972年从C3H小鼠胚胎细胞中分离建立；在同系小鼠体内不能成瘤，未发现自发转化；化学制剂很容易引起该细胞

发生转化，建议只用5~15代的细胞 。经无支原体鉴定入库。

动物种别/Organism 小鼠

组织来源 Tissue and Cell Type 胚胎

形态Morphology：成纤维细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培养基（货号：MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=4357355

Reznikoff C.A., Brankow D.W., Heidelberger C.

Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.

Cancer Res. 33:3231-3238(1973)

PubMed=4796800

Reznikoff C.A., Bertram J.S., Brankow D.W., Heidelberger C.

Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.

Cancer Res. 33:3239-3249(1973)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)