NCTC clone 929 [L cell, L-929, derivative of Strain L]小鼠胚胎成纤维细胞

  1948年三月建立了NCTC clone 929(小鼠结缔组织)，细胞系L的克隆。细胞系L是最早建立的连续培养细胞系之一，而clone 929是最早的克隆株。 从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。 第95代的细胞系L使用毛细管法分离单细胞建立了clone929。经无支原体鉴定入库。

动物种别/Organism 小鼠

组织来源Tissue and Cell Type 组织：皮下结缔组织；疏松结缔组织及脂肪 品系：

C3H/An

形态Morphology 成纤维细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：DMEM完全培养基（货号：MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications

Cells 2022, 11(3), 443; https://doi.org/10.3390/cells11030443

Collagen from Iris squid grafted with polyethylene glycol and collagen peptides promote the proliferation of fibroblast through PI3K/AKT and Ras/RAF/MAPK signaling pathways，https://doi.org/10.1016/j.ijbiomac.2023.125772

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)

PubMed=32218446; DOI=10.1038/s41597-020-0449-z

Zhong C.-Q., Wu J.-F., Qiu X.-F., Chen X., Xie C.-C., Han J.-H.

Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins.Sci. Data 7:104-104(2020)

Injectable Living Probiotic Dressing Built by Droplet-Based Microfluidics And Photo-Crosslinking to Prevent Pathogenic Infection And Promote Wound Repair

published: 16 October 2023

 https://doi.org/10.1002/adhm.202302423