P19小鼠畸胎瘤细胞

P19细胞株是从C3H/He小鼠中诱导的恶性畸胎瘤中建立的。该细胞在含有0.1mM2-巯基乙醇的培养基中可高效率地克隆。该细胞具有多能性，在500nM维A酸诱导下可以分化成神经和神经胶质样细胞；在0.5%~1.0%二甲亚砜(DMSO)存在下，分化形成心脏和骨骼肌样细胞，但不形成神经或神经胶质样细胞；在DMSO和维A酸同时存在时，细胞的分化与只有维A酸一样。
组织来源 Tissue and Cell Type： 胚胎；畸胎癌

形态Morphology：上皮细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions

90%αMEM(MD0800)+10%胎牛血清(SD0200)+150mg/L L-glutamine(SD0007)+G/A (SD0038)

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=7056443; DOI=10.1016/0012-1606(82)90338-4

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PLoS ONE 14:E0218412-E0218412(2019)