HEL299人胚肺成纤维细胞

      该细胞来源正常人男性胎儿；黑人，属于二倍体细胞，该细胞系为有限细胞株，传代有限。蛋白激酶C刺激后M2毒蕈碱受体表达下调。

     动物种别/Organism   人

     组织来源 Tissue and Cell Type 胚肺，成纤维细胞

     形态/Morphology 成纤维细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：MEM完全培养基（货号：MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=375235; DOI=10.1073/pnas.76.3.1288
Sherwin S.A., Sliski A.H., Todaro G.J.
Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.
Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979)

PubMed=6254071; DOI=10.1073/pnas.77.9.5258

Todaro G.J., Fryling C., De Larco J.E.

Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

Proc. Natl. Acad. Sci. U.S.A. 77:5258-5262(1980)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222

Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.

Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.

Am. Rev. Respir. Dis. 125:222-232(1982)