OK负鼠肾细胞

此细胞株最初是为研究X染色体失活时提供X染色体，随后发现它是极好的肾近曲小管上皮细胞的细胞培养模型。此细胞在培养时表达多种受体，并被广泛地用作研究这些受体的模型。    经无支原体鉴定入库。

动物种别/Organism  负鼠

组织来源 Tissue and Cell Type    肾近曲小管，上皮细胞

形态/Morphology 上皮细胞样；贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：MEM完全培养基（货号：MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=566717; DOI=10.1007/BF02616032

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In Vitro 14:239-246(1978)

CLPUB00465

Esposito D.

Cytogenetic and mutagenic studies with an opossum cell line (Didelphys virginiana).

Thesis PhD (1982), Fordham University, United States

PubMed=6394469; DOI=10.1007/BF02619628

Keith D.H., Teplitz R.L., Riggs A.D.

Metaphase synchronization and chromosome preparation from the OK opossum cell line having a potentially isolatable X chromosome.

In Vitro 20:833-836(1984)

PubMed=1802405; DOI=10.1016/0309-1651(91)90094-Y

Courjault F., Gerin B., Leroy D., Chevalier J., Toutain H.

Morphological and biochemical characterization of the opossum kidney cell line and primary cultures of rabbit proximal tubule cells in serum-free defined medium.

Cell Biol. Int. Rep. 15:1225-1234(1991)

PubMed=28615248; DOI=10.1152/ajprenal.00228.2017

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The transcriptome of the Didelphis virginiana opossum kidney OK proximal tubule cell line.

Am. J. Physiol. Renal Physiol. 313:F585-F595(2017)

PubMed=30379374; DOI=10.1113/EP087217

Guillen N., Caldas Y.A., Levi M., Sorribas V.

Identification and expression analysis of type II and type III Pi transporters in the opossum kidney cell line.

Exp. Physiol. 104:149-161(2019)