AtT-20小鼠垂体瘤细胞(分泌促生长激素分泌激素)

AtT20生长时不贴壁，而结成活细胞团。能抗脊髓灰质炎病毒。检测发现肢骨发育畸形病毒(鼠痘)阴性。 

动物种别/Organism  小鼠

组织来源 Tissue and Cell Type 垂体瘤细胞

形态/Morphology 小的圆形细胞，悬浮生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：1640完全培养基（货号：MD0201）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=13874682; DOI=10.1073/pnas.48.7.1184

Buonassisi V., Sato G., Cohen A.I.

Hormone-producing cultures of adrenal and pituitary tumor origin.

Proc. Natl. Acad. Sci. U.S.A. 48:1184-1190(1962)

PubMed=8383976; DOI=10.1677/jme.0.0100025

Farrell W.E., Stewart M.F., Clark A.J.L., Crosby S.R., Davis J.R.E., White A.

Glucocorticoid inhibition of ACTH peptides: small cell lung cancer cell lines are more resistant than pituitary corticotroph adenoma cells.

J. Mol. Endocrinol. 10:25-32(1993)

PubMed=15541569; DOI=10.1016/j.mce.2004.07.018

Ooi G.T., Tawadros N., Escalona R.M.

Pituitary cell lines and their endocrine applications.

Mol. Cell. Endocrinol. 228:1-21(2004)

PubMed=21214938; DOI=10.1186/1471-2164-12-14

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)