人BOB细胞系

这是一款，通过直肠穿刺活检(TRBP)建立自体永生化前列腺癌细胞系，该穿刺活检前列腺癌(CRPC)患者。Bob是CRPC功能研究，特别是CRPC“基础”表型研究的一种新的临床前模型。已知的现象是，由于癌细胞系的遗传不稳定性增加，Y染色体可能会重新排列或丢失，导致缺乏检测。根据STR-PCR分析，该细胞系与存款人提供的来源相同。

生长特性：鹅卵石样，贴壁生长

培养基：前列腺上皮细胞培养基

Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

培养和冻存方式：

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.

说明：产品仅供科研使用