携带GFP-hTert基因的人永生化视网膜微血管周细胞

背景介绍/context：

HRMVPCs are initiated by elutriation from dissociated normal human retinal tissue. Puromycin resistant GFP-hTert-HRMVPCs are selected from HRMVPCs after infected with lentiviruses expressing GFP and hTert. The cells are shipped in frozen vials (the cells are provided @ passage 4). Pericyte Growth Medium is recommended for cell culture and these cells have a minimum of population doubling capacity > 30 (tested so far) when cultured following the detailed protocol described below.

HRMVPCs是由分离的正常人视网膜组织的淘洗启动的。从HRMVPCs感染表达GFP和hTert的慢病毒后，选择具有普罗霉素抗性的GFP-hTert-HRMVPCs。细胞用冷冻小瓶运输(细胞见第4篇)。推荐周细胞生长培养基进行细胞培养，这些细胞至少具有增殖能力倍增的能力。按照以下详细的培养方案培养30例(目前已测试)。

动物种别/Organism  人

组织来源 Tissue and Cell Type 视网膜组织

形态/Morphology 贴壁生长 

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