小鼠永生化条件性骨细胞细胞系(IDG-SW3)

背景介绍/context：

IDG-SW3 represent a non-homogenous population progressing from early osteoblasts to late osteocytic. These cells express functional SV40 large T antigen that is induced in the presence of IFN γ under permissive temperature (33°C) and express GFP under control of the dentin matrix (Dmp1) promoter. Initial culture shows osteoblastic phenotype, however when under mineralizing conditions, the cells start to express early osteocyte markers such as E11/podoplanin, followed by Dmp1 and mature markers SOST and FGF23 by 21-28 days of culture. 

Similar to osteocytes in vivo, these cells respond to hormonal signals such as PTH and 1,25-dihydroxyvitamin-D3. IDG-SW3 cells can be maintained in both 2D and 3D cultures and are useful for study of osteocytes at various differentiation stages. It should be noted that these cultures may contain a mixture of osteoblasts and osteocytes at different stages of differentiation and for studies requiring enriched osteocytes, the Dmp1-GFP cells should be isolated by flow cytometry (FACS).

IDG-SW3代表一个从早期成骨细胞发展到晚期成骨细胞的非同质群体。这些细胞表达功能SV40大T抗原，该抗原是在允许温度下(33 C) IFN不表达的情况下诱导产生的，并在牙本质基质(Dmp1)启动子的控制下表达GFP。初始培养表现成骨细胞表型，但在矿化条件下，细胞开始表达早期骨细胞标志物，如E11/podoplanin，培养21-28天后表达Dmp1和成熟标志物SOST和FGF23。与体内骨细胞相似，这些细胞对激素信号如PTH和1,25-二羟维生素d3做出反应。IDG-SW3细胞可以在2D和3D培养中维持，对研究不同分化阶段的骨细胞很有用。值得注意的是，这些培养物可能包含不同分化阶段的成骨细胞和骨细胞的混合物，对于需要丰富骨细胞的研究，Dmp1-GFP细胞应该通过流式细胞术(FACS)分离出来。

动物种别/Organism  小鼠

组织来源 Tissue and Cell Type 成骨细胞

形态/Morphology 贴壁生长 

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