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CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. CD34+ stem cells are multipotent and can give rise to all cell types in hematopoietic system. Additionally, CD34 cells are responsible for all lymphohematopoietic lineages even though they comprise only a small portion of the cell population.Our Mobilized CD34+ cells (HSCs) are isolated from mobilized leuko paks using positive immunomagnetic cell separation procedures. All mobilized peripheral blood is collected in acid-citrate-dextrose formula A (ACDA) from fully consented IRB approved donors that have been mobilized with G-CSF (MPB).