IPSC从成纤维细胞与Niemann Pick型C -女性供体

背景介绍/context：

iPSC (induced Pluripotent Stem cells) reprogrammed from cells of a patient with Morbus Niemann Pick type C (NPC1 mutation)

Gender: Female;Age: 8 years 

Sibling to the donor of iPSC FFNPC-01

The iPS cell line was generated using an episomal reprogramming containing a prprietary mix of vectors, containing Oct-4, Sox-2, Klf-4 along with p53 Anti-sense, EBNA-1.

Cells are grown under feeder-free conditions and routinely grown in iPS Growth media. iPS cells are validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4.iPS colonies exhibit classical morphology and growth.Absence of Mycoplasma was confirmed.The cell line is shipped on dry ice.

C型Morbus Niemann Pick (NPC1突变)患者细胞重编程iPSC(诱导多能干细胞)

性别:女

年龄:8岁

与iPSC FFNPC-01供体同胞

iPS细胞系的使用一个包含prprietary混合游离重组生成的向量,包含Oct-4 Sox-2, Klf-4 p53反义,EBNA-1。细胞在无诱导条件下生长，并常规生长在iPS生长培养基中。基于集落形态、碱性磷酸酶表达和SSEA-4表达，iPS细胞被验证为多能性。iPS菌落呈现典型的形态和生长。证实无支原体。细胞系用干冰运送。

动物种别/Organism  人

组织来源 Tissue and Cell Type 多能干细胞

形态/Morphology 贴壁生长 

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