人间充质干细胞表达的OCT3/4

背景介绍/context：

It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent.

Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms.

Optimizing your hMSC Cultures: 

1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..

2) Use of our MSCGroTM media is highly recommended.

3) Subtrate- We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.

诱导多能干细胞的产生是通过共转染四种基因:Oct3/4、Sox2、Kfl4和c-Myc进入成体细胞，导致这些细胞重新编程，使其具有等同于胚胎干细胞的多能性。最近，包括我们实验室结果在内的独立研究表明，在成人神经或间充质干细胞中，单个基因Oct3/4的过度表达也会导致分化能力的增强，包括多能性。这一进展简化了iPS的产生，并可能反映了多能成体干细胞中Sox2、Kfl4和c-Myc的内源性表达水平。

此外，由于Oct3/4启动子具有众所周知的响应元件和转录控制机制，通过使用小分子试剂选择性激活Oct3/4的表达，基于环境线索的重新编程现在是可行的。我们研究的表达Oct3/4的人MSCs可以进一步研究iPSC机制。

优化你的hMSC培养:

1)在1 - 5% O2, 5% CO2中培养细胞，气体含量以N2平衡。

2)强烈推荐使用MSCGroTM媒体。

3)减量-我们推荐Greiner Bio-one tc处理的细胞培养瓶或同等的。

动物种别/Organism  人

组织来源 Tissue and Cell Type 多能干细胞

形态/Morphology 贴壁生长 

注意事项：此产品仅供科研使用