人间充质干细胞祖细胞

背景介绍/context：

Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. Cells have the capabilties to: 

Form adherent monolayers – ideal for 96- and 384-well formats for high throughput and high content cell-based assays.

To differentiate into a wide variety of mesenchymal subtypes, including osteogenic and chondrogenic lineages (but not adipogenic)– ideal for drug discovery for a wide variety of biological targets and basic research in bone, cartilage, metabolic and immunological diseases.

Passage up to 10X.

Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only.

Specifications: 

Cell Type: mesenchymal progenitor cells 

Species: human 

Source: derived from human embryonic cell line, H9 (WA09)

 Morphology: fibroblastic

Quantity: >1x10^6 cells per vial (viable post-thaw) 

Shipping method: dry ice

Storage: liquid nitrogen

Growth Properties: adherent

 proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine

 Phenotyping: Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. 

Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.

间充质干细胞祖细胞来源于人胚胎细胞系H9 (WA09)。细胞中CD90、CD105、CD166、CD73阳性占80%，Oct4阳性占5%。细胞具有以下能力:

形成贴壁单分子膜-理想的96孔和384孔格式的高通量和高含量的细胞为基础的分析。
分化为多种间充质亚型，包括成骨和软骨源性谱系(但不包括脂肪源性)，是发现多种生物靶点和骨、软骨、代谢和免疫疾病基础研究的理想药物。

传代可达到10个倍增。

注:请在订购时包括30美元的干冰费用。价格仅针对美国和加拿大。

规格:

细胞类型:间充质祖细胞

种类:人

来源:来自人类胚胎细胞系,H9 (WA09)

形态:成纤维细胞

数量:每瓶1x10^6细胞(解冻后活细胞)

运输方法:干冰

保存方法:液态氮增长

属性:贴壁生长 

增殖培养基:补充10%胎牛血清、Pen/Strep和l -谷氨酰胺的αMEM培养基

表现型:细胞中CD90、CD105、CD166、CD73阳性占80%，Oct4阳性占5%。

DNA指纹图谱:结果与单细胞的存在一致，没有培养交叉污染的证据。

病原体检测:该批次来自H9 hESC细胞系，已对乙型肝炎、丙型肝炎、HIV-1、HIV-2、HTLV-I/II、HSV1、HSV2、EBV和CMV进行检测。H9细胞系已经过测试，显示为阴性(由GIVF实验室进行)。还测试间充质细胞的无菌性和支原体污染。

注意事项：此产品仅供科研使用